Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture.

This guide describes general principles and considerations involved in western blotting using chemiluminescent substrates. The strategies and methods discussed are applicable to most enhanced chemiluminescence (ECL) western blot assays.

Download this free guide to discover:

• Types of considerations of chemiluminescence western blotting
• The factors that affect western blot results
• How to capture the elusive chemiluminescent signal
• Tips for optimizing your western blots