---
title: "The SG-NEx project: nanopore long-read RNA-sequencing of human cancer cell lines"
description: In this webinar I will introduce the SG-NEx project, compare the data obtained from the different RNA-Seq protocols, and show a comparison with short-read sequencing data.
image: https://go.technologynetworks.com/hubfs/STARLIMS-990x200-1.png
---

![Beyond-gene-expression_OnDemand-990x200](https://go.technologynetworks.com/hs-fs/hubfs/Technology%20Networks/TN%20Webinars/Oxford%20Nanopore%2028th%20Feb%202018/Beyond-gene-expression_OnDemand-990x200.png?width=990&name=Beyond-gene-expression_OnDemand-990x200.png "Beyond-gene-expression_OnDemand-990x200")

# Overview

Title:

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Duration:

Some additional information in one line

Avaliable On Demand

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# Webinar Summary

The ability of RNA-sequencing to generate a high-dimensional, quantitative readout of cells and tissues has made it arguably the most successful functional genomics assay in molecular biology. A new generation of long-read sequencing technology using nanopores enables amplification-free, direct sequencing of native RNA. The technology has the potential to overcome the major limitations of short-read sequencing, promising to provide a richer and more accurate readout of the cellular transcriptome. To systematically evaluate nanopore technology, we started the Singapore Nanopore-Expression Project (SG-NEx). As part of SG-NEx we generated full-length transcript sequencing data of 5 cancer cell lines using PCR-cDNA sequencing (PCR-cDNA), amplification-free cDNA sequencing (direct cDNA), and direct sequencing of native RNA (direct RNA) on the MinION, GridION, and PromethION platforms.

 In this webinar I will introduce the SG-NEx project, compare the data obtained from the different RNA-Seq protocols, and show a comparison with short-read sequencing data. I will include an overview of the computational workflow and highlight considerations for experimental design of a long-read transcriptomics experiment.

**Key Learning Objectives:**

- An introduction to the Singapore Nanopore-Expression Project (SG-NEx) and how to access the first batch of SG-NEx RNA-Seq data
- What to expect from the different RNA-Seq protocols from Oxford Nanopore (direct RNA vs cDNA vs PCR)
- How long-read RNA-Seq data compares to short-read RNA-Seq data

# Speaker Information:

![Jonathan Goke](https://go.technologynetworks.com/hs-fs/hubfs/Technology%20Networks/Landing%20Pages/TN%20Webinars/Oxford%20Nanopore%2028th%20Feb%202018/Jonathan.png?width=100&name=Jonathan.png "Jonathan Goke")

Jonathan Goke  
Senior Research Scientist (PI)   
Genome Institute of Singapore (A\*STAR)

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