Immunohistochemistry (IHC) is a popular tool used to advance our understanding of fundamental biology. The role of different cell types in the onset of diseases or in drug responses can also be directly observed. As powerful as IHC is, the way in which you should extract information from your images is not always obvious.
Here we expose 10 tips to help you design a strategy for analyzing your IHC images. These general tips are intended to help you design your analysis, and can be applied to your image analysis software of choice.