---
title: 3D Co-Culture Spheroids With Human Liver Cells
description: "Download this app note to learn more about:\n\nCo-culture liver spheroid formation\nHow to better understand the role of non-parenchymal cells\nFurther optimization of spheroid culture"
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---

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App Note

# 3D Co-Culture Spheroids With Human Liver Cells

More physiologically and pathologically relevant preclinical human liver models are needed for drug liver liability assessment and liver disease modeling. While Drug-Induced Liver Injury remains a major safety concern, several recent high-profile NAFLD/NASH clinical trial failures further highlight this critically unmet need. With the advances in 3D culture, liver spheroids made from primary human hepatocytes have been demonstrated to be a useful novel tool for *in vitro* hepatotoxicity studies.

**Download this app note to learn more about:**

- Co-culture liver spheroid formation
- How to better understand the role of non-parenchymal cells
- Further optimization of spheroid culture

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**Download this app note to learn more about:**

- Co-culture liver spheroid formation
- How to better understand the role of non-parenchymal cells
- Further optimization of spheroid culture

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