High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis.

In this technical note, we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS™ high-content analysis system, overcoming the most critical of challenges of 3D assays.

You will learn how to:

• Reduce the loss of image resolution as you image deeper into the sample
• Increase throughput for 3D imaging through shorter exposure times
• Enable 3D segmentation and analysis for your high-content assays
• Decrease the number of data transfer steps between software packages