High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis.
In this technical note, we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS™ high-content analysis system, overcoming the most critical of challenges of 3D assays.
You will learn how to:
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