---
title: 5 Key Steps to Successful CRISPRi [How to Guide]
description: This guide includes key steps on implementing CRISPRi using dCas9 derived from Streptococcus pyogenes.
---

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## 5 Key Steps to Successful CRISPRi \[How to Guide\]

The CRISPR interference (CRISPRi) machinery is an efficient, reversible and specific genome regulating platform for transcription control, without altering the target DNA sequence.

This guide includes key steps on implementing CRISPRi using dCas9 derived from *Streptococcus pyogenes.*

**Download this guide to learn:**

- Pros and Cons of CRISPRi
- How to plan and perform assys using CRISPRi
- How to avoid common mistakes in CRISPRi workflows

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*Complete the below form to download the full guide courtesy of Technology Networks.*

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