The CRISPR interference (CRISPRi) machinery is an efficient, reversible and specific genome regulating platform for transcription control, without altering the target DNA sequence.
This guide includes key steps on implementing CRISPRi using dCas9 derived from Streptococcus pyogenes.
Download this guide to learn:
- Pros and Cons of CRISPRi
- How to plan and perform assys using CRISPRi
- How to avoid common mistakes in CRISPRi workflows