ELISAs (Enzyme Linked Immunosorbent Assays) are a type of immunoassay that are commonly used to quantify levels of a specific target within a sample, usually run in 96-well microplates coated with a capture antibody specific for the analyte of interest. There are many different immunoassay platforms available to measure protein levels in biological fluids. However, ELISAs are preferred in many cases due to their sensitivity, specificity, accuracy and ability to tolerate harsh buffers or pretreatments.
- What’s at the core of your immunoassay?
- Why use an ELISA over other techniques?
- What is the importance of ELISA controls?