Since the introduction of Western blotting in the late 1970s, it has become a ubiquitous lab technique, enabling the analysis of protein expression in a wide variety of biological sample types.

Whether you’re a Western blot expert or just getting started, understanding the pros and cons of the available chemiluminescence- or fluorescence-based signal detection methods will let you make the right choice for your Western blot experiments.

In this technical note, we review common detection methods used in Western blotting to:

• help you understand your options, improve your workflow
• maximize your data
• how FluorChem imagers can give you maximum flexibility in your applications.