Advancing Top-Down Proteomics for Precision Medicine on the timsTOF Pro
The structure, activity, and function of proteins are modulated by posttranslational modifications (PTMs) which yield diverse molecular forms of individual expressed proteins. Read More Below
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Webinar Summary
To understand biological systems at a functional level, a comprehensive picture of the diverse forms proteins can take is crucial. Top-down mass spectrometry (MS)-based proteomics provides a comprehensive analysis of such proteoforms, but the extraordinary dynamic range of the human proteome has meant that the MS detection of low-abundance proteins has remained an unsolved challenge. New strategies in top-down proteomics for precision medicine enabled by the timsTOF Pro are however helping to change this.
In this webinar, David Roberts will present novel top-down proteomics and nanotechnology approaches for the sensitive enrichment, analysis, and characterization of low-abundance protein biomarkers.
Moreover, David will reveal a first-hand look at how the timsTOF Pro enables in-depth proteomics for the high-throughput proteomic analysis of 3D human induced pluripotent stem cell-derived engineered cardiac tissues assisted by a novel photo-cleavable surfactant, Azo. Lastly, David will demonstrate new native ion mobility MS approaches for the in-depth characterization of native antibody drug conjugates with high sensitivity.
- Top-down proteomics approaches for in-depth biomarker analysis toward precision medicine
- How rationally designed nanomaterials can enable the sensitive and specific enrichment of low-abundance proteins
- How TIMS enhances intact protein analysis and characterization and enables high-throughput, in-depth proteome coverage
- timsTOF Pro-enabled native MS characterization of antibody drug conjugates
Speaker Information:
Chemistry PhD Student
Ying Ge Lab and Song Jin Lab, University of Wisconsin-Madison
