App Note

Quantify 500 plasma proteins in 11.5 minutes using timsTOF pro with PASEF and 4D feature alignment


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The unique characteristics of the timsTOF Pro with PASEF allow routine measurements of ion mobility information and enable high quality 4D feature alignment in retention time, m/z and ion mobility (1/K0) and MS1 intensity.

Quantitiative analysis by immunoassays are limited in the number of proteins or analytes that can be measure simultaneously. Whilst mass spectrometry (MS)-based proteomics provides an attractive alternative, there are a number of challenges that make it difficult to implement.

Download this application note learn more about:

  • The limtiations of LC-MS/MS-based proteomics
  • How the timsTOF Pro with PASEF and Evosep One facilitates biomarker discovery in large sample cohorts