The practice of ultrasensitive MS-based proteomics has seen remarkable breakthroughs in the last few years. Improvements at different stages involved in the proteomics workflow have pushed sensitivity to a level which nowadays allows scientists to successfully identify and quantify more than 1000 proteins from a single mammalian cell.
While the development of more performant LC column technology has typically lagged behind, next generation pillar array columns are advancing proteome analysis in limited sample workflows.
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