Determining cell viability is a vital component in many biological experiments and one of the most common methods for this measurement is the trypan blue (TB) exclusion assay. Previous studies have shown that TB exclusion assays reported significantly higher viabilities when compared to fluorescence-based methods, but these studies have not provided evidence on potential reasons for the differences.
Download this whitepaper to learn how an image-based cytometry method was used to investigate:
- The effectiveness of TB in samples with low viability
- How light microscopy may affect results
- The differences between room temperature-incubated and heat-shocked cells