DIA vs DDA in Proteomics



Over recent years, liquid chromatography coupled to tandem mass-spectrometry (LC-MS/MS) has become the MS technology of choice for the high-throughput characterization of proteins and proteomes.

There are two modes of acquisition that can be used in LC-MS/MS experiments: the more traditional data dependent acquisition (DDA) mode, and the recently developed data independent acquisition (DIA) approaches, such as SWATH.

Download this infographic to gain an understanding of:

  • LC-MS/MS
  • The processes involved in an experiment using a DDA approach
  • The processes involved in an experiment using a DIA approach
  • How DIA produces a higher number of quantified proteins in a shorter anaylsis period than DDA

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