Handle With Care: Ensuring LNP Quality for Better Genetic Medicines

Now Available On Demand!

Lipid nanoparticles (LNPs) are widely adopted vehicles for oligonucleotide-based therapeutics and vaccines. LNPs are composed of helper lipids, PEGylated lipids, and ionizable lipids. Their detailed characterization and related impurities are of major importance for developing a successful product. Specifically, N-oxide-based impurities, leading to the loss in function of mRNA, pose a major concern.

In this webinar, Adam Crowe looks at how an ionizable lipid, commonly known as DLin-MC3-DMA (MC3), was analyzed with liquid chromatography coupled to mass spectrometry (LC-MS) using electron-activated dissociation (EAD), a novel type of low-energy electron-impact fragmentation. 

During this talk we discuss:

  • The localization of double bonds, their saturated impurities, and differentiation of different oxidated species, including N-oxide localization
  • The applicability of this method for other types of ionizable lipids and derived impurities
  • Which levels of N-oxides are problematic for mRNA efficacy

Speaker Information:

Adam Crowe, PhD
Precision NanoSystems Inc.
Analytical Development

Watch for FREE Now!