Having several crRNAs to guide the editing of the gene at different positions could result in a more thorough functional gene knockout, leading to a more robust phenotype that would improve hit identification. However, there are several potential concerns for using crRNA pools.
Download this app note to:
- Address concerns and learn about key considerations related to the use of crRNA pools
- Discover an example that shows pooling crRNAs to the same gene target provides robust functional knockout
- Reveal ways to ensure crRNA pools work successfully in phenotypic screening