Detecting Endogenous Nucleotides From Low Numbers of Mammalian Cells
Biomedical laboratories are under increasing pressure to produce meaningful data from minute amounts of sample. In this webinar, we’ll explore how a micro-scale separation technique, capillary electrophoresis-mass spectrometry (CE-MS), can be used for the highly efficient and sensitive profiling of nucleotides from a low number of mammalian cells.
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Webinar Summary
Biomedical laboratories are under increasing pressure to produce meaningful data from minute amounts of sample. In this webinar, we’ll explore how a micro-scale separation technique, capillary electrophoresis-mass spectrometry (CE-MS), can be used for the highly efficient and sensitive profiling of nucleotides from a low number of mammalian cells.
Endogenous nucleotides can be detected in extracts as low as 500 HepG2 cells. In a research study discussed in this webinar, an injection volume of 6.5 nL resulted in sub-nanomolar detection limits for nucleotides, clearly illustrating the utility of this technique for metabolic profiling in low amounts of biological material.
- How an electro-driven separation can help detection of endogenous nucleotides with as few as 500 HepG2 cells
- Review the separation principle and key application areas of capillary electrophoresis-mass spectrometry (CE-MS)
- Discover the potential of CE-MS for metabolomics studies with limited sample volumes
Speaker Information:
Principal Investigator
Leiden University
