Full spectrum flow cytometry is becoming increasingly popular as it offers novel fluorescent dye combinations, improving flexibility and increasing multicolor panel size. This webinar explains the differences between spectral and conventional flow cytometry and considers best practice for spectral flow cytometry. Fluorescent dye selection, controls, sample preparation and panel design will be discussed, helping you to generate optimal data.
We also demonstrate that Bio-Rad’s new StarBright Dyes have exceptional brightness, unique spectral profiles and stability and high lot-to-lot reproducibility, making them ideal for spectral flow cytometry. We show how their unique profiles enable novel dye combinations and the creation of larger panels of increased complexity.
- Fluorophore requirements for spectral flow cytometry
- Best practice for panel building in spectral flow cytometry
- StarBright Dyes, their properties and how they can improve multicolor panels
- How StarBright Dyes can help to build larger, more complex panels