Fluorescent Western Blotting — An Introduction for New Users [Application Note]


Western blotting is an important technique in life science research. While most life science researchers use chemiluminescent substrates for detection, more scientists are now taking advantage of fluorescence detection because of an increased need for multiplexing.

With the introduction of advanced digital imaging instruments and improvements in fluorescent conjugate technologies, scientists now have the necessary tools to take advantage of the range of fluorescent dyes and antibodies for western blot detection.

Download this application note to learn:

  • Best practices to help enable your success with fluorescent western blotting
  • Considerations for optimal fluorescence detection
  • How to accurately normalize western blots
  • How to troubleshoot common issues