Tech Note

How Strandedness is maintained during library preparation



RNA sequencing (RNA-Seq) data are growing in importance for the understanding of disease onset and progression. The ability to detect the strand of genome encoding the gene transcript, known as Stranded RNA-Seq, has been shown to more accurately quantify gene expression levels. However, the process is not without its challenges.

Download this technical note to find out:

  • The techniques used in RNA-Seq
  • The challenges in RNA-Seq workflows
  • How to produce libraries that are 98% stranded