Identifying Oligonucleotide Impurities and Metabolites With High-Resolution Mass Spectrometry

With the rapid increase in the development of oligonucleotide therapeutics and gene therapies, workflows for the identification, characterization and quantification of full-length products, their impurities and metabolites, are critical. High-resolution time-of-flight mass spectrometry (TOF-MS) can be used for this analysis offering several advantages over ligand-binding assays or fluorescence-based liquid chromatography. Notably, TOF-MS can provide simultaneous identification and sequence confirmation for drug substances as well as impurities/metabolites.

In this webinar, a novel LC-MS/MS Q-TOF instrument (ZenoTOF 7600 system, SCIEX) equipped with a patented ion trap will be applied to the acquisition of highly modified oligonucleotides. Furthermore, the advanced data processing capabilities of a novel software application (Molecule Profiler software, SCIEX) will be demonstrated for their ability to enable identification and relative quantification of synthetic impurities as well as metabolites of oligonucleotide therapeutics.

Attend this webinar to:

• Explore how accurate mass, isotope patterns and enhancements in MS/MS are employed to find potential impurities/metabolites
• Learn how impurity/metabolite ID can be automated and how structural characterization based on MS/MS spectra can be facilitated
• Discover how oligonucleotide impurity and metabolite analysis using LC-MS/MS can be performed with Molecule Profiler software

Speaker Information: