Immunofluorescence (IF) is a powerful method for visualizing proteins expressed directly within tissues. The IF method combines immunology and fluorescent molecules to localize proteins within defined morphological structures, and thus, provides insights into gene expression, protein–protein interactions and biomarker identification. This method is used in a wide variety of applications, including basic research, assessment of normal and disease states in human and animal health, as well as in plant pathology studies.
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