Understanding the role of distinct cellular phenotypes in tissue function, development, and pathogenesis requires tools that can rapidly and consistently quantify the expression of multiple proteins while preserving spatial information. To meet this need, we have developed Multiplexed Ion Beam Imaging by Time-Of-Flight (MIBI-TOF).
MIBI-TOF uses secondary ion mass spectrometry to visualize up to 50 metal labeled antibodies at subcellular resolution simultaneously with histochemical stains and native biological elements. We have used this capability to study how single cell function and tissue structure interact at the tumor immune microenvironment, the human maternal-fetal interface, and in infectious disease.
- MIBI-TOF and its applications in understanding the tumor immune microenvironment and other disease processes
- Recent developments in creating brighter primary ion sources and new metal reporter constructs
