WEBINAR

LC-MS Meets MS Imaging: Using CCS for Confident Lipid Annotation

May 26, 2021 | 10AM EDT | 3PM BST | 4PM CEST
WebinarsBY-TN-White

 

 

The involvement of lipids in many biological and physiological processes makes their analysis of paramount importance. High-resolution mass spectrometry (HR-MS) combined with fragmentation experiments can identify lipid species. Read More Below

Brought to you by

bruker_logo

Register Today!

Webinar Summary

The involvement of lipids in many biological and physiological processes makes their analysis of paramount importance. High-resolution mass spectrometry (HR-MS) combined with fragmentation experiments can identify lipid species. However, to differentiate isomeric lipids or isobaric interferences, powerful additional separation techniques, often (ultra)high performance liquid chromatography, are required.

Trapped ion mobility-mass spectrometry (TIMS) for the separation of ions after matrix-assisted laser desorption/ionization (MALDI) has become a useful tool in lipid research, increasing the reliability of lipid identification using complementary collision cross section (CCS) data. 

In this study, we created a tissue-specific CCS database using hydrophilic interaction liquid chromatography coupled to electrospray TIMS-MS. With the help of this database, we were able to distinguish isomeric lipid classes clearly, such as phosphatidylglycerol (PG) and its regio-isomer bis(monoacylgylcero)phosphate (BMP), as well as isobaric interferences due to adduct formation during the MALDI process. The developed workflow and the application to a mouse spleen sample for confident lipid identification applying MALDI-TIMS-MS imaging will be presented.

In this webinar, you will learn how to: 
  • Use trapped ion mobility separation to identify isomeric lipids confidently
  • Separate BMP and PG lipids using TIMS
  • Use CCS to visualize confident lipid annotations spatially on tissue
  • Build tissue-specific CCS databases

Speaker Information:


WebinarSpeakerImage
Heiko Hayen
Professor for Analytical Chemistry
University of Münster, Germany

 

 

 

Following our interaction with you, Bruker Daltonics and its affiliates within the Bruker group (“Bruker”) would like to be able to stay in contact with you and inform you about the latest technological achievements and upcoming events as well as the products and services offered by Bruker. If you are interested in hearing from us in the future, we will need your consent in accordance with applicable data protection laws to be allowed to engage in marketing activities using your contact information.

To view the promises that we make to you regarding your personal information, please view Bruker`s privacy policy. For questions related to privacy, please send your inquiry to privacy@bruker.com

If you prefer not to receive promotional e-mails from Bruker in the future, you can unsubscribe at any time here.

Copyright © 2020 Bruker Scientific LLC | 40 Manning Road | Billerica, MA | 01821.
For research use only. Not for use in diagnostic procedures.