Mass spectrometry imaging (MSI) of clinical samples can be daunting due to the presence of high abundance, uninteresting molecules in the tissues or due to the way that the samples are processed and preserved. For example, the presence of hemoglobin from red blood cells can suppress the signal from other, lower abundance proteins that may be more relevant to the disease being studied.
A second challenge is that most clinical tissue samples are banked as formalin fixed, paraffin embedded tissue specimens, which present difficulties for the detection and imaging of biomolecules.
In this webinar, we will present new sample preparation strategies for overcoming these issues and producing high quality MSI data.
- A basic introduction to MSI
- An overview of challenges related to MSI of clinical samples
- Instructions for sample preparation techniques for reduction of hemoglobin and for imaging metabolites from formalin fixed, paraffin embedded tissue sections
- Insights on the applications of new sample preparation strategies for clinical samples