Alterations in metabolic flux have been linked to a wide variety of cell processes, from cancer development to T cell activation during immunotherapy or infection.
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Alterations in metabolic flux have been linked to a wide variety of cell processes, from cancer development to T cell activation during immunotherapy or infection. This talk will cover two complementary technologies for measuring metabolic flux: Seahorse XF analyzers and liquid chromatography-mass spectrometry (LC/MS) qualitative flux analysis. Both technologies provide important insights into the rates of metabolic reactions within cells, with Seahorse XF measurements providing cellular resolution, and LC/MS qualitative flux measurements providing molecular resolution.
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