Multiple Focused CRISPR Libraries from a Single Oligo Pool

CRISPR Libraries

twist-bioscience cropped


CRISPR Libraries

Functional genetic screening using the CRISPR/Cas9 system is a powerful tool for interrogating gene function at scale, but access to and implementation of this technology is constrained by existing pre-defined CRISPR sgRNA libraries. Fully custom sgRNA libraries solve this issue by allowing tunability and flexibility in a CRISPR screen.

Download this app note to discover a custom sgRNA library design that:

  • Can be split into multiple sub-pools with ease, each with tightly uniform distribution 
  • Can be used for focused genomic interrogations without wasted screening, saving time and money