Temperature cycling is fundamental to all PCR reactions; precise temperature control by a thermal cycler is crucial for the accuracy and efficiency of the reaction. However, a multitude of thermal cycler instruments exist, each with varying capabilities; this can make it difficult to know which one is best suited to your experiments.
This series of app notes can help you evaluate published ramp rates, temperature uniformity, and temperature accuracy for leading thermal cycler models, covering key aspects such as:
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