Setting up live cell experiments can be challenging, as temperature, CO2 and evaporation need to be carefully controlled to ensure optimal cell growth conditions.

In this technical note, we monitor evaporation rates, cell growth and morphology inside the temperature- and CO2-controlled live cell chamber of the Operetta CLS™ high-content analysis system and compare the results with those obtained using a humidity-controlled cell culture incubator.

Learn how you can:

• Perform successful five-day live cell imaging on Operetta CLS and Opera Phenix™ high-content systems.
• Avoid phototoxicity with gentle digital phase contrast imaging.
• Analyze cell growth and morphology on a single cell level without fluorescence staining