Reconstructing plant genomes is a difficult task due to their unusual ploidy, the large number of repetitive elements present and they are often large in size. Sequencing technologies that are capable of producing long reads, such as Oxford Nanopore’s, show promise for obtaining more complete and accurate genome assemblies. The first critical step here is the extraction of high-molecular weight DNA using the appropriate protocol. In the case of homozygous genomes, this technology can generate contigs that represent complete chromosomes and reveal the architecture of complex regions, like centromeres, rDNA clusters, or clusters of paralogous genes.
In this presentation, Caroline will focus on the sequencing of a banana genome, and the reconstruction of complete chromosomes from telomere to telomere using nanopore sequencing technology.
- Generating gapless, telomere-to-telomere plant genome assemblies with nanopore technology
- Using long and ultra-long nanopore reads to resolve highly repetitive sequences