The purity of oligonucleotide preparations produced by solid-phase synthesis can be very high. However, purification is often required to remove incomplete or erroneous sequences.
Anion exchange chromatography (AIEX) is an attractive choice since it can separate the negatively charged oligonucleotides based on length. The obtained purity and yield of the full-length oligonucleotide is dependent on the chromatographic resolution as well as the binding and elution conditions.
Comparing WorkBeads™ 40Q with one of the market leading resins proved: