Real-time, High Density Monitoring of pTyr Signaling Targets in Human Tumors Using Heavy Peptide Triggered Targeted Quantitation



Post-translational modification (PTM) signaling networks can govern cellular deregulation and many diseases. Efforts to understand these networks have harnessed LC-MS to catalog several thousand PTM sites across many functional protein categories.

Protein tyrosine phosphorylation (pTyr) is a PTM of great interest; it is highly correlated to human kinase oncogenes, despite only accounting for <1% of total phosphorylation events.

Download this poster note to discover a standardized workflow for monitoring tyrosine phosphorylation networks, including:

  • The technologies used
  • How it was established
  • Its advantages over traditional approaches