Biopharmaceuticals are trending towards high concentration therapeutics, but with higher concentrations also comes an increased risk of protein aggregation. The diffusion interaction parameter (kD) and the second virial coefficient (B22) are well-established parameters for predicting the colloidal stability and the aggregation propensity of proteins. These parameters can be determined independently of each other using Dynamic Light Scattering (DLS) and Static Light Scattering (SLS).
This application note describes how you can compare the aggregation propensity of different protein formulations with the B22 and kD application.
Download this application note to discover how Stunner offers a unique combination of simultaneous protein quantification and sizing and can: