Immunoassays are a broad family of biochemical tests united by their use of antibody–antigen binding to detect given targets. This may mean using an experimental antibody as a tool to detect the presence of a specific protein, such as a nuclear protein in a cell sample. Conversely, it may involve using a recombinant protein to enable the detection of natural antibodies within a sample, for example when looking for seroconversion to a pathogen within a population.
Irrespective of the specific technique chosen, there are some factors common to all, that could and should be considered when looking to develop, use or troubleshoot an immunoassay.
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Choosing the most appropriate antibodies and buffers
