---
title: Tips for Successful Immunoassays
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HOW TO GUIDE

# Tips for Successful Immunoassays

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![ImmunoassayMokeuo](https://go.technologynetworks.com/hs-fs/hubfs/Technology%20Networks/TN%20Editorial%20Calendar/How%20To%20Guides/2021/Immunoassay/ImmunoassayMokeuo.png?width=600&height=376&name=ImmunoassayMokeuo.png "ImmunoassayMokeuo")

Immunoassays are a broad family of biochemical tests united by their use of antibody–antigen binding to detect given targets. This may mean using an experimental antibody as a tool to detect the presence of a specific protein, such as a nuclear protein in a cell sample. Conversely, it may involve using a recombinant protein to enable the detection of natural antibodies within a sample, for example when looking for seroconversion to a pathogen within a population.

Irrespective of the specific technique chosen, there are some factors common to all, that could and should be considered when looking to develop, use or troubleshoot an immunoassay.

**Download this how-to guide for tips on:**

- Choosing the most appropriate antibodies and buffers
- Maintaining sample quality
- Washing techniques
- Detecting multiple targets
- Monitoring assay performance

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