Polymerase chain reaction (PCR) is a very popular technique for amplifying nucleic acids, with a wide range of applications and variations. Even those who perform PCR routinely will come across errors from time to time, therefore a full understanding of the principles of PCR, an awareness of common errors and lots of practice will help in improving the accuracy and reproducibility of results.
This guide offers advice, tips, troubleshooting notes and more, making this an essential read for anyone who performs PCR and wants to improve on their technique.
Download this guide to learn about the PCR protocol, plus how to troubleshoot problems including:
- No product
- Faint product
- Incorrect or nonspecific product
- Sequence errors