Ultrapure Water for HPLC Analysis

High Performance Liquid Chromatography (HPLC) is an analytical procedure for separation, identification and quantification of substances using liquid chromatography (LC). The mixture for separation is transferred to a column with a solvent (eluting agent) or with a solvent mixture (eluant/mobile phase), by an injector and a pump. Among the typical applications for HPLC is sugar analysis.

Eluants must have especially high physical and chemical purity, and may not contain suspended mechanical particles or any dissolved substances to avoid noise on chromatograms. Sartorius has demonstrated how their arium® pro VF provides water of the quality required for good separations and clean chromatograms. Among the typical applications for HPLC is sugar analysis, which is used as an example in this guide.

This app note demonstrates how arium® pro VF can be used in HPLC to purify water by:

• Providing an overview of the importance of good quality eluants
• Describing the arium® pro VF and how it works
• Relaying the materials and method used to successfully separate sugar mixtures using ultrapure water from the arium® pro VF
• Concluding with results and a summary of applications