Vitamin D Biomarker Quantitation in Plasma: Improving Limits of Detection

Vitamin D is widely monitored and used as an indicator for skeletal health in both children and adults with deficiencies resulting in rickets and osteoporosis. Vitamin D2 and vitamin D3 are hydroxylated in the liver to form 25-hydroxyvitamin D2 (OH-Vit D₂) and 25-hydroxyvitamin D3 (OH-Vit D₃). Measurement of these metabolites is the preferred method for assessing total vitamin D due to their high circulating concentrations (ng/mL) and serum half-life.

Solid-phase extraction (SPE) is an efficient technique to separate critical analytes – such as OH-Vit D₂ and OH-Vit D₃ – that are dissolved or suspended in a liquid mixture according to their physical and chemical properties.

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• Unparalleled performance compared to conventional SPE, phospholipid, and protein precipitation methods
• Simple extraction of OH-Vit D2 and OH-Vit D3 from human plasma
• Increased reproducibility and sensitivity
• Reduced sample and solvent requirements