You’ve read the protocol a hundred times, you’ve tried your lab mate’s suggestions and left various offerings to the western blotting Gods – but it’s just no good. Your western blot still looks a mess, the bands don’t appear like the ones in the paper or perhaps you can’t see any bands at all!
Help is at hand! Download this guide to discover tips and tricks on:
- Preparation and optimization
- Selecting the right controls
Blocking and washing